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Addgene

pGEX Rad S273A
(Plasmid #11233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4950
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rad
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    S273A
  • Entrez Gene
    RRAD (a.k.a. RAD, RAD1, REM3)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX Rad S273A was a gift from Ronald Kahn (Addgene plasmid # 11233 ; http://n2t.net/addgene:11233 ; RRID:Addgene_11233)

  • For your References section:

    Effects of phosphorylation on function of the Rad GTPase. Moyers JS, Zhu J, Kahn CR. Biochem J. 1998 Aug 1. 333 ( Pt 3)():609-14. PubMed 9677319
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