pTEAD1-donor
(Plasmid
#112329)
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PurposeCRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMB1 ori
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTEAD1 homology arms flanking EGFP-IRES-Neo cassette
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SpeciesH. sapiens (human)
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Entrez GeneTEAD1 (a.k.a. AA, NTEF-1, REF1, TCF-13, TCF13, TEAD-1, TEF-1)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning source: hg19 reference. Use with plasmid pTEAD1.1.0-gDNA.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTEAD1-donor was a gift from Kevin White (Addgene plasmid # 112329 ; http://n2t.net/addgene:112329 ; RRID:Addgene_112329)
