pLL3.7-EF-EYFP-YAP1_delta5C-PolyA
(Plasmid #112289)

• Purpose: Fluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the protein
• Depositing Lab: Erik Sahai
• Publication: Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13. doi: 10.1016/j.cels.2018.05.006. Epub 2018 Jun 18.

Backbone

• Vector backbone: pLentilox3.7
• Backbone size w/o insert (bp): 7484
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EYFP-YAP1
• Species: H. sapiens (human)
• Insert Size (bp): 2462
• Mutation: Human YAP1-2gamma lacking the last 5 Amino acids (FLTWL) at the C-terminus
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: EF-1 alpha

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human YAP1-2gamma isoform, which is 504 amino acids long is described in (Gaffney et al., 2012)