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pLL3.7-EF-EYFP-YAP1_Y3F-PolyA
(Plasmid #112288)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentilox3.7
  • Backbone size w/o insert (bp) 7484
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EYFP-YAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2462
  • Mutation
    3 Tyrosines (341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in human YAP1-2gamma) to Phenylalanine
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Promoter EF-1 alpha

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The human YAP1-2gamma isoform, which is 504 amino acids long is described in (Gaffney et al., 2012)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL3.7-EF-EYFP-YAP1_Y3F-PolyA was a gift from Erik Sahai (Addgene plasmid # 112288 ; http://n2t.net/addgene:112288 ; RRID:Addgene_112288)

  • For your References section:

    Quantitative Analysis Reveals that Actin and Src-Family Kinases Regulate Nuclear YAP1 and Its Export. Ege N, Dowbaj AM, Jiang M, Howell M, Hooper S, Foster C, Jenkins RP, Sahai E. Cell Syst. 2018 Jun 27;6(6):692-708.e13. doi: 10.1016/j.cels.2018.05.006. Epub 2018 Jun 18. 10.1016/j.cels.2018.05.006 PubMed 29909276
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