pGEX Rad C249
(Plasmid
#11228)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11228 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-2T
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4950
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRad
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SpeciesH. sapiens (human)
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Insert Size (bp)800
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MutationC249: deletion of amino acids 249-308
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Entrez GeneRRAD (a.k.a. RAD, RAD1, REM3)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The C249 mutant contains a deletion of the calmodulin binding site.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX Rad C249 was a gift from Ronald Kahn (Addgene plasmid # 11228 ; http://n2t.net/addgene:11228 ; RRID:Addgene_11228) -
For your References section:
Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II. Moyers JS, Bilan PJ, Zhu J, Kahn CR. J Biol Chem. 1997 May 2. 272(18):11832-9. 10.1074/jbc.272.18.11832 PubMed 9115241