CMV-PKCg-mEGFP-N1
(Plasmid
#112270)
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PurposeIDOCKSgamma Donor Construct
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112270 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemEGFP-N1
- Backbone size w/o insert (bp) 4718
- Total vector size (bp) 6809
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameprotein kinase c gamma
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Alt namePKCg
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Alt namePKCgamma
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Alt nameprkcg
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SpeciesM. musculus (mouse)
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Insert Size (bp)2091
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Entrez GenePrkcg (a.k.a. PKCgamma, Pkcc, Prkcc)
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer cmv
- 3′ sequencing primer GFP_N (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV-PKCg-mEGFP-N1 was a gift from Ryohei Yasuda (Addgene plasmid # 112270 ; http://n2t.net/addgene:112270 ; RRID:Addgene_112270) -
For your References section:
PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine BDNF signaling to facilitate synaptic plasticity. Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, Yasuda R. Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16. 10.1038/s41593-018-0184-3 PubMed 30013171