CMV-PKCa-mEGFP-N1
(Plasmid #112269)

• Purpose: IDOCKSalpha Donor Construct
• Depositing Lab: Ryohei Yasuda
• Publication: Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16.

Backbone

• Vector backbone: mEGFP-N1
• Backbone size w/o insert (bp): 4718
• Total vector size (bp): 6734
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Protein Kinase C alpha
• Alt name: PRKCa
• Species: B. taurus (bovine)
• Insert Size (bp): 2016
• Mutation: A11V (please see depositor comments below)
• GenBank ID: AAI51472.1
• Entrez Gene: PRKCA (a.k.a. PKRCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: cmv
• 3′ sequencing primer: GFP_N

Resource Information

• Supplemental Documents:
  • CMV_PKCa_GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that mutation A11V was found in PKCa during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.