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Addgene

CMV-2mCh-PS
(Plasmid #112268)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112268 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mEGFP-C1
  • Modifications to backbone
    mEGFP replaced by mCh
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    two copies of mCherry followed by a pseudosubstrate peptide for PKC
  • Alt name
    2mCh_PS
  • Species
    Synthetic
  • Insert Size (bp)
    1510
  • Promoter CMV
  • Tag / Fusion Protein
    • mCh (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-2mCh-PS was a gift from Ryohei Yasuda (Addgene plasmid # 112268 ; http://n2t.net/addgene:112268 ; RRID:Addgene_112268)

  • For your References section:

    PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine BDNF signaling to facilitate synaptic plasticity. Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, Yasuda R. Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16. 10.1038/s41593-018-0184-3 PubMed 30013171
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