Skip to main content
Addgene

CMV-mEGFP-PKCa-C1
(Plasmid #112264)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    mEGFP-C1
  • Backbone size w/o insert (bp) 4650
  • Total vector size (bp) 6699
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    protein kinase C alpha
  • Alt name
    PKCalpha
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2016
  • Mutation
    A11V (please see depositor comments below)
  • GenBank ID
    AAI51472.1
  • Entrez Gene
    PRKCA (a.k.a. PKRCA)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that mutation A11V was found in PKCa during Addgene's quality control. The depositor noted that this mutation does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-mEGFP-PKCa-C1 was a gift from Ryohei Yasuda (Addgene plasmid # 112264 ; http://n2t.net/addgene:112264 ; RRID:Addgene_112264)

  • For your References section:

    PKCalpha integrates spatiotemporally distinct Ca(2+) and autocrine BDNF signaling to facilitate synaptic plasticity. Colgan LA, Hu M, Misler JA, Parra-Bueno P, Moran CM, Leitges M, Yasuda R. Nat Neurosci. 2018 Aug;21(8):1027-1037. doi: 10.1038/s41593-018-0184-3. Epub 2018 Jul 16. 10.1038/s41593-018-0184-3 PubMed 30013171
Related items:
Commonly requested with: