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Addgene

pU6-EF-gAAVS1
(Plasmid #112262)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112262 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Total vector size (bp) 3338
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    g-AAVS1
  • gRNA/shRNA sequence
    gtcaccaatcctgtccctag
  • Species
    Synthetic
  • Promoter U6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-EF-gAAVS1 was a gift from Zhili Rong (Addgene plasmid # 112262 ; http://n2t.net/addgene:112262 ; RRID:Addgene_112262)

  • For your References section:

    iKA-CRISPR hESCs for inducible and multiplex orthogonal gene knockout and activation. Ma S, Lv J, Sun J, Tang P, Li H, Zhou H, Zhang Z, Lin Y, Rong Z. FEBS Lett. 2018 Jun 5. doi: 10.1002/1873-3468.13127. 10.1002/1873-3468.13127 PubMed 29869798
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