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Addgene

pBABE Rad 39-297
(Plasmid #11226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rad
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    760
  • Mutation
    N39/C297: deletion of amino acids 1-39 and 298-308
  • Entrez Gene
    RRAD (a.k.a. RAD, RAD1, REM3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This mutant begins at Rad 2nd ATG. Made because in vivo basal phosphorylation of Rad appears to be in residues 1-39 and 298-308, so theoretically this mutant should not be phosphorylated basally in cells.)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE Rad 39-297 was a gift from Ronald Kahn (Addgene plasmid # 11226 ; http://n2t.net/addgene:11226 ; RRID:Addgene_11226)

  • For your References section:

    Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II. Moyers JS, Bilan PJ, Zhu J, Kahn CR. J Biol Chem. 1997 May 2. 272(18):11832-9. 10.1074/jbc.272.18.11832 PubMed 9115241
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