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pcDNA3.1_C-6His-Stx17
(Plasmid #112259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+C-6His
  • Backbone manufacturer
    Genescript
  • Backbone size w/o insert (bp) 5432
  • Total vector size (bp) 6347
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    syntaxin 17
  • Alt name
    STX17
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    915
  • GenBank ID
  • Entrez Gene
    Stx17 (a.k.a. 4833418L03Rik, 6330411F21Rik, 9030425C21Rik, AW048351)
  • Promoter T7
  • Tag / Fusion Protein
    • 6His Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (unknown if destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGgg
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_C-6His-Stx17 was a gift from Madhulika Tripathi (Addgene plasmid # 112259 ; http://n2t.net/addgene:112259 ; RRID:Addgene_112259)
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