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Addgene

pET28a-SpyCatcher-mi3
(Plasmid #112255)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112255 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher-mi3
  • Species
    Synthetic
  • Insert Size (bp)
    960
  • Promoter T7
  • Tag / Fusion Protein
    • C-tag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SpyCatcher-mi3 was a gift from Mark Howarth (Addgene plasmid # 112255 ; http://n2t.net/addgene:112255 ; RRID:Addgene_112255)

  • For your References section:

    Engineering a Rugged Nanoscaffold to Enhance Plug-and-Display Vaccination. Bruun TUJ, Andersson AC, Draper SJ, Howarth M. ACS Nano. 2018 Jul 20. doi: 10.1021/acsnano.8b02805. 10.1021/acsnano.8b02805 PubMed 30028591
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