p87AAVEGFP hp47phox (S303/304/328D)
(Plasmid
#112250)
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Purposemutant p47phox
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112250 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep87
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namep47phox
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SpeciesH. sapiens (human)
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Insert Size (bp)1170
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MutationS303/304/328D
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Entrez GeneNCF1 (a.k.a. CGD1, NCF1A, NOXO2, SH3PXD1A, p47phox)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p87AAVEGFP hp47phox (S303/304/328D) was a gift from Simone Di Giovanni (Addgene plasmid # 112250 ; http://n2t.net/addgene:112250 ; RRID:Addgene_112250)