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pcDNA3. 3xFlag:PP4-19c[D85NH115N]
(Plasmid #112247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PP4
  • Species
    D. melanogaster (fly)
  • Mutation
    D85NH115N
  • Entrez Gene
    Pp4-19C (a.k.a. Dmel_CG32505, CG1459, CG1596, CG18339, CG32505, DmPpp4-19C, Dmel\CG32505, PP1, PP4, PP4 19C, PP4-19C, PP4-19c, PP4C, PP4c, PPP4c, PPX, PpX, Ppp4c, dPP4, pp4)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3. 3xFlag:PP4-19c[D85NH115N] was a gift from Simone Di Giovanni (Addgene plasmid # 112247 ; http://n2t.net/addgene:112247 ; RRID:Addgene_112247)
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