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pUWL201PW-creDEM
(Plasmid #112242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUWL201PW

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Shuttle vector. Ampicillin resistance in E. coli. Thiostrepton resistance in Streptomyces.
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    creD
  • GenBank ID
    ALA99201.1

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer GCACGCGGTCGATCTTGACGGCT
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    creE
  • GenBank ID
    ALA99202.1

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer GCACGCGGTCGATCTTGACGGCT
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    CreM
  • GenBank ID
    ALA99210.1

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUWL201PW-creDEM was a gift from Emily Balskus (Addgene plasmid # 112242 ; http://n2t.net/addgene:112242 ; RRID:Addgene_112242)

  • For your References section:

    Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis. Waldman AJ, Balskus EP. J Org Chem. 2018 May 29. doi: 10.1021/acs.joc.8b00367. 10.1021/acs.joc.8b00367 PubMed 29771512
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