Skip to main content
Addgene

pUWL201PW-creM-N-His6
(Plasmid #112240)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112240 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUWL201PW

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Streptomyces/E. coli shuttle vector. Ampicillin in E. coli. Thiostrepton in Streptomyces.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    creM
  • GenBank ID
    ALA99210.1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer GCACGCGGTCGATCTTGACGGCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUWL201PW-creM-N-His6 was a gift from Emily Balskus (Addgene plasmid # 112240 ; http://n2t.net/addgene:112240 ; RRID:Addgene_112240)

  • For your References section:

    Discovery of a Diazo-Forming Enzyme in Cremeomycin Biosynthesis. Waldman AJ, Balskus EP. J Org Chem. 2018 May 29. doi: 10.1021/acs.joc.8b00367. 10.1021/acs.joc.8b00367 PubMed 29771512
Related items:
Commonly requested with: