(+)-Limonene Synthase
(Plasmid
#112230)
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Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112230 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name(+)-Limonene Synthase
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SpeciesCitrus sinensis
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Mutationdeleted amino acids 1-52
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Tag
/ Fusion Protein
- N-terminal His-tag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
(+)-Limonene Synthase was a gift from Daniel Oprian (Addgene plasmid # 112230 ; http://n2t.net/addgene:112230 ; RRID:Addgene_112230) -
For your References section:
Functional and Structural Characterization of a (+)-Limonene Synthase from Citrus sinensis. Morehouse BR, Kumar RP, Matos JO, Olsen SN, Entova S, Oprian DD. Biochemistry. 2017 Mar 28;56(12):1706-1715. doi: 10.1021/acs.biochem.7b00143. Epub 2017 Mar 15. 10.1021/acs.biochem.7b00143 PubMed 28272875
