gfp-Arkadia/ RNF111 deltaRING
(Plasmid #112229)

• Purpose: Expresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cells
• Depositing Lab: Vasso Episkopou
• Publication: Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.1371/journal.pbio.0050067.

Backbone

• Vector backbone: pTriEx2-hygro
• Backbone manufacturer: Novagen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: (Arkadia)Rnf111
• Alt name: Arkadia
• Alt name: Rnf111
• Species: M. musculus (mouse)
• Mutation: delta RING domain deletion (Deletion of the last 24 aa that included the second Zinc finger of the RING domain).
• Entrez Gene: Rnf111 (a.k.a. ARK, Arkadia)
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SmaI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Arkadia gene originated from Episkopou et al 2001; DOI: 10.1038/35071095

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The GFP tagged Arkadia constructs were generated by eliminating the first 9 aa of Arkadia and fusing in frame to GFP Sequences and cloned into SmaI/XhoI of the pTriEx2-hygro (Novagen, http://www.novagen.com) vector.