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gfp-Arkadia/RNF111
(Plasmid #112228)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112228 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx2-hygro
  • Backbone manufacturer
    Novagen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    (Arkadia)Rnf111
  • Alt name
    Arkadia
  • Alt name
    Rnf111
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rnf111 (a.k.a. ARK, Arkadia)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pCAG-F
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Arkadia gene originated from Episkopou et al 2001; DOI: 10.1038/35071095

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The GFP tagged Arkadia constructs were generated by eliminating the first 9 aa of Arkadia and fusing in frame to GFP Sequences and cloned into SmaI/XhoI of the pTriEx2-hygro (Novagen, http://www.novagen.com) vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gfp-Arkadia/RNF111 was a gift from Vasso Episkopou (Addgene plasmid # 112228 ; http://n2t.net/addgene:112228 ; RRID:Addgene_112228)

  • For your References section:

    Arkadia enhances Nodal/TGF-beta signaling by coupling phospho-Smad2/3 activity and turnover. Mavrakis KJ, Andrew RL, Lee KL, Petropoulou C, Dixon JE, Navaratnam N, Norris DP, Episkopou V. PLoS Biol. 2007 Mar;5(3):e67. doi: 10.1371/journal.pbio.0050067. 10.1371/journal.pbio.0050067 PubMed 17341133
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