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Addgene

pET30a-RAMA
(Plasmid #112226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112226 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30a
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5850
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MPND
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Mpnd (a.k.a. E130307M08Rik)
  • Tags / Fusion Proteins
    • HIs S-tag (N terminal on backbone)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a-RAMA was a gift from Douglas Feldman (Addgene plasmid # 112226 ; http://n2t.net/addgene:112226 ; RRID:Addgene_112226)

  • For your References section:

    An Adversarial DNA N(6)-Methyladenine-Sensor Network Preserves Polycomb Silencing. Kweon SM, Chen Y, Moon E, Kvederaviciute K, Klimasauskas S, Feldman DE. Mol Cell. 2019 Apr 5. pii: S1097-2765(19)30222-9. doi: 10.1016/j.molcel.2019.03.018. 10.1016/j.molcel.2019.03.018 PubMed 30982744
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