pMTB
(Plasmid
#112225)
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PurposeExpression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMTB
- Total vector size (bp) 7217
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Vector typeZebrafish Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuper-folder GFP
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Alt namesfGFP
- Promoter beta actin 2 (zebrafish)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer M13 Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMTB was a gift from Sean Megason (Addgene plasmid # 112225 ; http://n2t.net/addgene:112225 ; RRID:Addgene_112225) -
For your References section:
Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo. Wagner DE, Weinreb C, Collins ZM, Briggs JA, Megason SG, Klein AM. Science. 2018 Apr 26. pii: science.aar4362. doi: 10.1126/science.aar4362. 10.1126/science.aar4362 PubMed 29700229