pcDNA-3xflagGREB1a
(Plasmid
#112221)
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Purposemammalian expression of flag tagged GREB1a
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112221 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1 + hygro
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGREB1a
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SpeciesH. sapiens (human)
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Insert Size (bp)5846
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Entrez GeneGREB1
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Tag
/ Fusion Protein
- 3x flag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 or CMV
- 3′ sequencing primer BGH R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-3xflagGREB1a was a gift from Craig Burd (Addgene plasmid # 112221 ; http://n2t.net/addgene:112221 ; RRID:Addgene_112221) -
For your References section:
GREB1 isoforms regulate proliferation independent of ERalpha co-regulator activities in breast cancer. Haines CN, Braunreiter KM, Mo XM, Burd CJ. Endocr Relat Cancer. 2018 Jul;25(7):735-746. doi: 10.1530/ERC-17-0496. Epub 2018 Apr 25. 10.1530/ERC-17-0496 PubMed 29695586
Map uploaded by the depositor.
