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pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH
(Plasmid #112212)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112212 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-terminus of dCas9 and DNMT3A
  • Species
    H. sapiens (human)
  • Mutation
    H840A,N863A
  • Entrez Gene
    DNMT3A (a.k.a. DNMT3A2, HESJAS, M.HsaIIIA, TBRS)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH was a gift from Xingxu Huang (Addgene plasmid # 112212 ; http://n2t.net/addgene:112212 ; RRID:Addgene_112212)

  • For your References section:

    Locus-specific DNA methylation of Mecp2 promoter leads to autism-like phenotypes in mice. Lu Z, Liu Z, Mao W, Wang X, Zheng X, Chen S, Cao B, Huang S, Zhang X, Zhou T, Zhang Y, Huang X, Sun Q, Li JD. Cell Death Dis. 2020 Feb 3;11(2):85. doi: 10.1038/s41419-020-2290-x. 10.1038/s41419-020-2290-x PubMed 32015323
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