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Addgene

pBABE Rad S273A
(Plasmid #11221)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rad
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Mutation
    S273A
  • Entrez Gene
    RRAD (a.k.a. RAD, RAD1, REM3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a mutation of an in vitro site of phosphorylation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE Rad S273A was a gift from Ronald Kahn (Addgene plasmid # 11221 ; http://n2t.net/addgene:11221 ; RRID:Addgene_11221)

  • For your References section:

    Effects of phosphorylation on function of the Rad GTPase. Moyers JS, Zhu J, Kahn CR. Biochem J. 1998 Aug 1. 333 ( Pt 3)():609-14. PubMed 9677319
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