pANY6
(Plasmid
#112198)
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Purpose(Empty Backbone) This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAny6
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Backbone manufacturerYingfeng An
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Vector typeBacterial Expression
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Tag
/ Fusion Protein
- ELP-tag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pANY6 was a gift from Yingfeng An (Addgene plasmid # 112198 ; http://n2t.net/addgene:112198 ; RRID:Addgene_112198) -
For your References section:
Three Novel Escherichia coli Vectors for Convenient and Efficient Molecular Biological Manipulations. Gao H, Qi X, Hart DJ, Gao S, Wang H, Xu S, Zhang Y, Liu X, Liu Y, An Y. J Agric Food Chem. 2018 Jun 20;66(24):6123-6131. doi: 10.1021/acs.jafc.8b01960. Epub 2018 Jun 11. 10.1021/acs.jafc.8b01960 PubMed 29798665
Map uploaded by the depositor.
