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Addgene

MCV-HF LTS220A/S239A
(Plasmid #112191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ241
  • Backbone manufacturer
    DNA2.0
  • Backbone size w/o insert (bp) 2755
  • Total vector size (bp) 8142

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCV-HF LTS220A/S239A
  • Species
    Merkel cell polyomavirus
  • Insert Size (bp)
    5387
  • Mutation
    T to G mutations at 3313 (nt) and 3370 (nt) of full construct
  • GenBank ID
    JF813003
  • Promoter None from vector. Insert contains native promoter from viral genome encompassed in the viral non-coding control region.

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCV-HF LTS220A/S239A was a gift from Patrick Moore (Addgene plasmid # 112191 ; http://n2t.net/addgene:112191 ; RRID:Addgene_112191)

  • For your References section:

    Protein-mediated viral latency is a novel mechanism for Merkel cell polyomavirus persistence. Kwun HJ, Chang Y, Moore PS. Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4040-E4047. doi: 10.1073/pnas.1703879114. Epub 2017 May 1. 10.1073/pnas.1703879114 PubMed 28461484
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