Aahn
(Plasmid
#112158)
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PurposeA genetically encoded voltage indicator (Aahn), capable of optically monitoring both plasma membrane and internal membrane potentials.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112158 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5600
- Total vector size (bp) 7100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAahn
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SpeciesCiona
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Insert Size (bp)1500
- Promoter CMV
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Tag
/ Fusion Protein
- Super ecliptic pHlorin A227D (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Aahn was a gift from Bradley Baker (Addgene plasmid # 112158 ; http://n2t.net/addgene:112158 ; RRID:Addgene_112158) -
For your References section:
Monitoring voltage fluctuations of intracellular membranes. Sepehri Rad M, Cohen LB, Braubach O, Baker BJ. Sci Rep. 2018 May 2;8(1):6911. doi: 10.1038/s41598-018-25083-7. 10.1038/s41598-018-25083-7 [pii] PubMed 29720664
