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Addgene

jk089_h12
(Plasmid #112151)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112151 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    peAA.11 and peDK.11
  • Total vector size (bp) 11827

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    Room Temperature
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    IMPORTANT: Grow at room temperature to prevent recombination!
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    C. thermophilum DASH/Dam1 complex
  • Alt name
    peAA.11.c_the.dash_003.peDK.11.c_the.dash_012
  • Tags / Fusion Proteins
    • his tag on Spc34 (C terminal on insert)
    • strep tag on Dad1 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Screening multiple colonies/gel purification of plasmid may be required due to recombination potential of plasmid

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    jk089_h12 was a gift from Stephen Harrison (Addgene plasmid # 112151 ; http://n2t.net/addgene:112151 ; RRID:Addgene_112151)

  • For your References section:

    Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface. Jenni S, Harrison SC. Science. 2018 May 4;360(6388):552-558. doi: 10.1126/science.aar6436. 10.1126/science.aar6436 PubMed 29724956
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