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Addgene

LUC45 (L/Sec/mutP)
(Plasmid #112148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core
  • Species
    R. norvegicus (rat); Photinus pyralis
  • Mutation
    Cysteine 258 mutated to selenocysteine (U) and SECIS AUGA to AUCC
  • Entrez Gene
    Gpx4 (a.k.a. Gshpx-4, Phgpx, gpx-4, snGpx)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LUC45 (L/Sec/mutP) was a gift from Paul Copeland (Addgene plasmid # 112148 ; http://n2t.net/addgene:112148 ; RRID:Addgene_112148)

  • For your References section:

    Efficiency of mammalian selenocysteine incorporation. Mehta A, Rebsch CM, Kinzy SA, Fletcher JE, Copeland PR. J Biol Chem. 2004 Sep 3;279(36):37852-9. doi: 10.1074/jbc.M404639200. Epub 2004 Jun 30. 10.1074/jbc.M404639200 PubMed 15229221
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