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Addgene

LUC56 (Luc/UGA/SP)
(Plasmid #112147)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112147 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR
  • Species
    R. norvegicus (rat); Photinus pyralis
  • Mutation
    Cysteine 258mutated to selenocysteine (U)
  • Entrez Gene
    Selenop (a.k.a. Sepp1)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LUC56 (Luc/UGA/SP) was a gift from Paul Copeland (Addgene plasmid # 112147 ; http://n2t.net/addgene:112147 ; RRID:Addgene_112147)

  • For your References section:

    Regulation of selenocysteine incorporation into the selenium transport protein, selenoprotein P. Shetty SP, Shah R, Copeland PR. J Biol Chem. 2014 Sep 5;289(36):25317-26. doi: 10.1074/jbc.M114.590430. Epub 2014 Jul 25. 10.1074/jbc.M114.590430 PubMed 25063811
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