pBABE Rad S105N
(Plasmid #11214)

• Depositing Lab: Ronald Kahn
• Publication: Zhu et al J Biol Chem. 1996 Jan 12. 271(2):768-73.

Backbone

• Vector backbone: pBABE-puro
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rad
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: S105N
• Entrez Gene: RRAD (a.k.a. RAD, RAD1, REM3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A potential dominant negative mutant of Rad, devoid of GTP-binding activity in vitro. The first in-frame ATG of rad is utilized in vivo. Hence, full-length Rad comprises 308 amino acid residues. The potentially dominant negative mutant of Rad (previously termed S66N-Rad) is therefore S105N-Rad.