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Addgene

pBABE Rad
(Plasmid #11213)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rad
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    RRAD (a.k.a. RAD, RAD1, REM3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE Rad was a gift from Ronald Kahn (Addgene plasmid # 11213 ; http://n2t.net/addgene:11213 ; RRID:Addgene_11213)

  • For your References section:

    Rad, a novel Ras-related GTPase, interacts with skeletal muscle beta-tropomyosin. Zhu J, Bilan PJ, Moyers JS, Antonetti DA, Kahn CR. J Biol Chem. 1996 Jan 12. 271(2):768-73. 10.1074/jbc.271.2.768 PubMed 8557685
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