pBABE-bleo IGF-1R
(Plasmid #11212)

• Depositing Lab: Ronald Kahn
• Publication: Entingh-Pearsall et al J Biol Chem. 2004 Sep 3. 279(36):38016-24.

Backbone

• Vector backbone: pBabe-bleo
• Backbone size w/o insert (bp): 4888
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IGF-1R
• Alt name: IGFR
• Alt name: IGF-I receptor
• Species: H. sapiens (human)
• Insert Size (bp): 4200
• Entrez Gene: IGF1R (a.k.a. CD221, IGFIR, IGFR, JTK13)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Eva Surmacz
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert was cloned from pcDNA IGF-1R (which contains a 4.2kb IGF-1R insert that lacks about 700bp of 3' sequence. This insert was cloned into the XhoI-XbaI sites of pcDNA). An EcoRI/XbaI fragment of pcDNA-IGF-1R was then inserted into SnaBI site of pBABE-bleo (EcoRI and XbaI sites were blunted).