pET-45b(+)-Tn5
(Plasmid #112112)

• Purpose: Expresses hyperactive Tn5 transposase for next generation sequencing library preparation
• Depositing Lab: Frank Pugh
• Publication: Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi: 10.1038/s41467-018-05265-7.

Backbone

• Vector backbone: pET-45b(+)
• Backbone size w/o insert (bp): 5260
• Total vector size (bp): 6703
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tn5
• Insert Size (bp): 1443
• Mutation: E54K E110K P242A L372P
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GenScript
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry