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pBABE-bleo human Insulin Receptor A
(Plasmid #11210)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11210 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabe-bleo
  • Backbone size w/o insert (bp) 4888
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human insulin receptor-isoform A
  • Alt name
    IR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4300
  • Mutation
    Lacks exon 11.
  • Entrez Gene
    INSR (a.k.a. CD220, HHF5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer pBABE 5'
  • 3′ sequencing primer pBABE 3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Ingo Leibiger

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HindIII/AflII of pRcCMVi-HIR-A (blunted) was ligated into BamHI (blunted) of pBABE-bleo.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-bleo human Insulin Receptor A was a gift from Ronald Kahn (Addgene plasmid # 11210)

  • For your References section:

    Bi-directional regulation of brown fat adipogenesis by the insulin receptor. Entingh AJ, Taniguchi CM, Kahn CR. J Biol Chem. 2003 Aug 29. 278(35):33377-83. 10.1074/jbc.M303056200 PubMed 12807888
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