pCMV_BE4max_P2A_GFP
(Plasmid #112099)

• Purpose: C:G-to-T:A base editing
• Depositing Lab: David Liu
• Publication: Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.

Backbone

• Vector backbone: pCMV
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BE4max_P2A_GFP
• Species: H. sapiens (human)
• Insert Size (bp): 6378
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: BGH-Reverse

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results indicate a mixed population at position 5224. The majority of the population is the correct G nucleotide, although a minority shows an A at this position. Scientists may want to sequence this region of the plasmid prior to performing experiments.