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Addgene

pCMV_ABEmax_3xHA
(Plasmid #112098)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112098 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABEmax_3xHA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5499
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV_ABEmax_3xHA was a gift from David Liu (Addgene plasmid # 112098 ; http://n2t.net/addgene:112098 ; RRID:Addgene_112098)
  • For your References section:

    Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP, Raguram A, Liu DR. Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172. 10.1038/nbt.4172 PubMed 29813047