pGEX SH3(2)-3R
(Plasmid
#112091)
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PurposeBacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX2T
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5700
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Modifications to backboneA Tev protease site has been added between the thrombin site and the multiple cloning site. The multiple cloning has been modified to begin with a NdeI (CATATG) site, and end with a BamHI (GGATCC) site.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSH3(2)-3R
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Alt nameNCK adaptor protein 1, isoform 2, second SH3 repeat x 3
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SpeciesH. sapiens (human)
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Insert Size (bp)702
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GenBank IDNP_001177725.1
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Entrez GeneNCK1 (a.k.a. NCK, NCKalpha, nck-1)
- Promoter Lac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3'
- 3′ sequencing primer pGEX-3’: 5'-CCGGGAGCTGCATGTGTCAGAGG-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX SH3(2)-3R was a gift from Michael Rosen (Addgene plasmid # 112091 ; http://n2t.net/addgene:112091 ; RRID:Addgene_112091) -
For your References section:
Phase transitions in the assembly of multivalent signalling proteins. Li P, Banjade S, Cheng HC, Kim S, Chen B, Guo L, Llaguno M, Hollingsworth JV, King DS, Banani SF, Russo PS, Jiang QX, Nixon BT, Rosen MK. Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879. 10.1038/nature10879 PubMed 22398450