pMal_Abl-PRM 3R
(Plasmid #112086)

• Purpose: Bacterial expression plasmid containing His and MBP tags for 3 PRM motif repeats of Human Abl.
• Depositing Lab: Michael Rosen
• Publication: Li et al Nature. 2012 Mar 7;483(7389):336-40. doi: 10.1038/nature10879.

Backbone

• Vector backbone: pMal-tev
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 6600
• Total vector size (bp): 6885
• Modifications to backbone: tev sites added on both sides of MCS, His-6 after c-terminal tev site
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Abl-PRM-3R
• Alt name: 3 repeats of the protein rich motif from proto-oncogene tyrosine-protein kinase (ABL)
• Species: H. sapiens (human)
• Insert Size (bp): 285
• Mutation: construct contains only the PRM motif of Abl1
• GenBank ID: AH005332.2 AAB60394
• Entrez Gene: ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
• Promoter: Lac
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • His-6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: pMAL-Fwd: 5'-TGCGTACTGCGGTGATCAAC-3'
• 3′ sequencing primer: pMAL-Rev: 5'-CTGCAAGGCGATTAAGTTGG-3'

Resource Information

• Supplemental Documents:
  • MTTH, modified pMal vector

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry