pKC-4.04
(Plasmid #112085)

• Purpose: Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin
• Depositing Lab: James Inglese
• Publication: Baird et al Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.

Backbone

• Vector backbone: p3xFLAG-CMV-10
• Backbone size w/o insert (bp): 6204
• Total vector size (bp): 8717
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3XFLAG firefly luciferase-beta-globin
• Alt name: FLuc NMD sensor control
• Species: H. sapiens (human), M. musculus (mouse); Photinus pyralis
• Insert Size (bp): 2513
• Entrez Gene: HBB (a.k.a. CD113t-C, ECYT6, beta-globin)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hgH-PA-R

Resource Information

• Supplemental Documents:
  • pKC-4.04
• A portion of this plasmid was derived from a plasmid made by: b-globin sequence used in this clone was obtained from Professor Lynne Maquat
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry