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Addgene

pcDNA-HA-Nedd4(C744E)/2797
(Plasmid #11206)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11206 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nedd4-1
  • Alt name
    Nedd4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Mutation
    cysteine 744 mutated to glutamic acid
  • GenBank ID
    U96635
  • Entrez Gene
    Nedd4 (a.k.a. E430025J12Rik, EG639396, Gm7265, KIAA0093, Nedd4-1, Nedd4a)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not I (not destroyed)
  • 3′ cloning site EcoR I (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HA-Nedd4(C744E)/2797 was a gift from Allan Weissman (Addgene plasmid # 11206 ; http://n2t.net/addgene:11206 ; RRID:Addgene_11206)

  • For your References section:

    WW domain HECT E3s target Cbl RING finger E3s for proteasomal degradation. Magnifico A, Ettenberg S, Yang C, Mariano J, Tiwari S, Fang S, Lipkowitz S, Weissman AM. J Biol Chem. 2003 Oct 31. 278(44):43169-77. 10.1074/jbc.M308009200 PubMed 12907674
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