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Addgene

ACTB-(A)2x
(Plasmid #112056)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112056 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACTB
  • Alt name
    NCBI Reference Sequence: NM_001101.3
  • Species
    H. sapiens (human)
  • Mutation
    2 copies of Riboglow tag (variant A) after stop codon
  • Entrez Gene
    ACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
  • Promoter CMV
  • Tag / Fusion Protein
    • Riboglow RNA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer Quintara Bio: CMV Forward (5'-CGCAAATGGGCGGTAGGCGTG-3')
  • 3′ sequencing primer Quintara Bio: SV50pA-R (5'-GAAATTTGTGATGCTATTGC-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2 copies of Riboglow (variant A), separated by restriction sites as follows: KpnI - A - KpnI - A - BamHI

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ACTB-(A)2x was a gift from Amy Palmer (Addgene plasmid # 112056 ; http://n2t.net/addgene:112056 ; RRID:Addgene_112056)

  • For your References section:

    A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells. Braselmann E, Wierzba AJ, Polaski JT, Chrominski M, Holmes ZE, Hung ST, Batan D, Wheeler JR, Parker R, Jimenez R, Gryko D, Batey RT, Palmer AE. Nat Chem Biol. 2018 Jul 30. pii: 10.1038/s41589-018-0103-7. doi: 10.1038/s41589-018-0103-7. 10.1038/s41589-018-0103-7 PubMed 30061719
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