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Addgene

hPrp16 C-terminus
(Plasmid #11205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX2TK
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4970
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Prp16 C-terminus
  • Alt name
    Prp16
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    840
  • Mutation
    Amino acids 948-1227
  • Entrez Gene
    DHX38 (a.k.a. DDX38, PRP16, PRPF16, RP84)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reed lab clone #355.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPrp16 C-terminus was a gift from Robin Reed (Addgene plasmid # 11205 ; http://n2t.net/addgene:11205 ; RRID:Addgene_11205)

  • For your References section:

    Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing. Zhou Z, Reed R. EMBO J. 1998 Apr 1. 17(7):2095-106. 10.1093/emboj/17.7.2095 PubMed 9524131
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