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Addgene

pHvL1P3GA
(Plasmid #112027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Synthetic
  • Backbone manufacturer
    Unknown
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4500
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LacZ
  • gRNA/shRNA sequence
    To be inserted
  • Tag / Fusion Protein
    • None

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHvL1P3GA was a gift from Wendy Harwood (Addgene plasmid # 112027 ; http://n2t.net/addgene:112027 ; RRID:Addgene_112027)

  • For your References section:

    Creating Targeted Gene Knockouts in Barley Using CRISPR/Cas9. Lawrenson T, Harwood WA. Methods Mol Biol. 2019;1900:217-232. doi: 10.1007/978-1-4939-8944-7_14. 10.1007/978-1-4939-8944-7_14 PubMed 30460568
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