hTex1-GST
(Plasmid
#11199)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11199 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX
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Backbone manufacturerAmersham
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTex1
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SpeciesH. sapiens (human)
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Insert Size (bp)1580
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GenBank IDBC006849
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Entrez GeneTHOC3 (a.k.a. THO3, hTREX45)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Reed lab clone #786.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hTex1-GST was a gift from Robin Reed (Addgene plasmid # 11199 ; http://n2t.net/addgene:11199 ; RRID:Addgene_11199) -
For your References section:
Recruitment of the human TREX complex to mRNA during splicing. Masuda S, Das R, Cheng H, Hurt E, Dorman N, Reed R. Genes Dev. 2005 Jul 1. 19(13):1512-7. 10.1101/gad.1302205 PubMed 15998806