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Addgene

Hsh155 N4A 3xFLAG
(Plasmid #111975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS414
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 5208
  • Total vector size (bp) 8208
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Hsh155 N4A 3x FLAG
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3000
  • Mutation
    T178A R181A R182A R186A
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hsh155 N4A 3xFLAG was a gift from Aaron Hoskins (Addgene plasmid # 111975 ; http://n2t.net/addgene:111975 ; RRID:Addgene_111975)

  • For your References section:

    Functional Analysis of Hsh155/SF3b1 Interactions with the U2 snRNA/Branch Site Duplex. Carrocci TJ, Paulson JC, Hoskins AA. RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118. 10.1261/rna.065664.118 PubMed 29752352
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