pJB02
(Plasmid #111959)

• Purpose: Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments
• Depositing Lab: Matthew Good
• Publication: Caldwell et al Biochemistry. 2018 May 8;57(18):2590-2596. doi: 10.1021/acs.biochem.8b00131. Epub 2018 Apr 19.

Backbone

• Vector backbone: pETARA
• Backbone size w/o insert (bp): 4346
• Total vector size (bp): 5592
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: mEGFP
• Alt name: GFP
• Insert Size (bp): 717
• Mutation: enhanced GFP with monomerizing A206K mutation
• Promoter: T7
• Tag / Fusion Protein:
  • DHFR (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Gene/Insert 2

• Gene/Insert name: eDHFR
• Alt name: Dehydrofolate reductase; DHFR
• Species: E. Coli
• Insert Size (bp): 474
• Mutation: eDHFR;residues 2-159
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Resource Information

• Supplemental Documents:
  • pJB02.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry