Hsh155 N747A
(Plasmid
#111949)
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PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the N747A mutation
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS414
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 4788
- Total vector size (bp) 8124
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHsh155 N747A
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3339
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MutationN747A
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Hsh155 N747A was a gift from Aaron Hoskins (Addgene plasmid # 111949 ; http://n2t.net/addgene:111949 ; RRID:Addgene_111949) -
For your References section:
Functional Analysis of Hsh155/SF3b1 Interactions with the U2 snRNA/Branch Site Duplex. Carrocci TJ, Paulson JC, Hoskins AA. RNA. 2018 May 11. pii: rna.065664.118. doi: 10.1261/rna.065664.118. 10.1261/rna.065664.118 PubMed 29752352
