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pGL4.26_4xUAS_DSCP_GFP
(Plasmid #111931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 111931 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4
  • Backbone manufacturer
    Promega
  • Total vector size (bp) 5207
  • Modifications to backbone
    We cloned the ubiquitin63E promoter (from pRL-ubi63E; Addgene ID 74280) upstream of the Gal4-DBD between the KpnI and BglII sites. The 4xUAS-GFP reporter plasmid was derived from pSGE_91_4xUAS_dCP (Stampfel et al, 2015) (Addgene ID 71169) by replacing the luciferase gene with sgGFP (Arnold et al, 2013).
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    4xUAS GFP

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The ubiquitin63E promoter was cloned from pRL-ubi63E (Addgene ID 74280) 4xUAS-GFP reporter plasmid was derived from pSGE_91_4xUAS_dCP (Stampfel et al, 2015) (Addgene ID 71169)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.26_4xUAS_DSCP_GFP was a gift from Alexander Stark (Addgene plasmid # 111931 ; http://n2t.net/addgene:111931 ; RRID:Addgene_111931)

  • For your References section:

    A high-throughput method to identify trans-activation domains within transcription factor sequences. Arnold CD, Nemcko F, Woodfin AR, Wienerroither S, Vlasova A, Schleiffer A, Pagani M, Rath M, Stark A. EMBO J. 2018 Aug 15;37(16). pii: embj.201798896. doi: 10.15252/embj.201798896. Epub 2018 Jul 13. 10.15252/embj.201798896 PubMed 30006452
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